liquid protein formulations are prone to form aggregates. the effect of nonionic surfactants such as polysorbate 20 (ps 20) and n-dodecyl β-d-maltoside (ddm) on the prevention of aggregation and conformational changes of recombinant human ifnβ-1b (rhifn β_1b) was explored. polysorbate has been used in formulations of protein pharmaceuticals. there have been concerns about using ps 20 due to its...

thermal conformational changes in human serum albumin (hsa) in present with a 10 mm phosphate buffer, at ph=7 have been investigated via circular dichroism (cd) and uv spectroscopic methods. the results indicate that temperature in a range of 25oc to 55oc could induce a reversible conformational change in the structure of hsa. the hsa phase transition corresponds to the physiological and pathol...

Plasmonic chirality has drawn much attention because of tunable circular dichroism (CD) and the enhancement for chiral molecule signals. Although various mechanisms have been proposed to explain the plasmonic CD, a quantitative explanation like the ab initio mechanism for chiral molecules, is still unavailable. In this study, a mechanism similar to the mechanisms associated with chiral molecule...

the surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. in this study, conformational changes of glucose oxidase (gox) in the mercaptopurine: gnps and 11-mercaptoundecanoic acid: gnps as a hydrophobic and a hydrophilic gnps surface was investigated by various spectroscopic techniques, including: uv-vis absorption, f...

A user-friendly website for the analysis of protein secondary structures from Circular Dichroism (CD) and Synchrotron Radiation Circular Dichroism (SRCD) spectra has been created.

hsa plays an important role in transporting metabolites and drugs throughout the vascular system.  in as much as its performance is very vital in the presents of different kinds of ligands at the specific body temperatures, its examination is crucial. this molecule can undergo increased glycation in diabetes. therefore, glucose as the one of the most fundamental ligands dealing with albumin in ...

Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years. With the advent of synchrotron radiation circular dichroism (SRCD) and improvements in instrumentation for conventional CD, lower wavelength data are obtainable and the information content of the spectra increased. In addition, new computation and bioinformatics metho...

The centrifugal liquid membrane (CLM) cell has been utilized for chiroptical studies of liquid-liquid interfaces with a conventional circular dichroism (CD) spectropolarimeter. These studies required the characterization of optical properties of the rotating cylindrical CLM glass cell, which was used under the high speed rotation. In the present study, we have measured the circular and linear d...

Bayesian modelling capturing uncertainty and correlations in circular dichroism (CD) spectra suggests it is not possible to identify more than 3 distinct secondary structure classes from CD above 175 nm.

Core-resonant circular dichroism (CD) signals are induced by molecular chirality and vanish for achiral molecules and racemic mixtures. The highly localized nature of core excitations makes them ideal probes of local chirality within molecules. Simulations of the circular dichroism spectra of several molecular families illustrate how these signals vary with the electronic coupling to substituti...